TABLE 2.
Primers used in this study
| Name | Sequencea | |
|---|---|---|
| 1 | hisG 1/24 (Sac1) | AGGAGCTCATGTTAGACAACACCCGCTTACGC |
| 2 | hisG 100/72 (TPS) | CGTCTCTGGCTCTATCGTAAAGGTACCAATTAATTTTTATGCCGCAGCGGGCCAG |
| 3 | hisG 803/834 (TPS) | TCACCGTATAGGATATCGGATCCTCAGCAGCGAAACGTTGTTCTGGGAAACCATG |
| 4 | hisG 900/879 (Sph1) | TCGCATGCTCACTCCATCATCTTCTCGATC |
| 5 | TPS1 1021/1059 (hisG) | GCGGCATAAAAATTAATTGGTACCTTTACGATAGAGCCAGAGACGATGCCGACGAAGATGAAG |
| 6 | TPS1 3684/3656 (hisG) | ACAACGTTTCGCTGCTGAGGATCCGATATCCTATACGGTGAAAAAACTACTTGCGCAG |
| 7 | SP-TPS1-T304G-A305T | CGATGAAATCGTCGACTTACACGTCAACGGGTTCAGTAATTCTATT |
| 8 | ASP-TPS1-T304G-A305T | AATAGAATTACTGAACCCGTTGACGTGTAAGTCGACGATTTCATCG |
| 9 | SP-TPS1-G332C | ACAACGGGTCCAGTAATTCTATTCTATCGCCGTTATTCCATT |
| 10 | ASP-TPS1-G332C | AATGGAATAACGGCGATAGAATAGAATTACTGGACCCGTTGT |
| 11 | SP-TPS1-A467G | CGATTTAACCTAGGTGCATGGTTACCATTTGATGTTGGTTC |
| 12 | ASP-TPS1-A467G | GAACCAACATCAAATGGTAACCATGCACCTAGGTTAAATCG |
| 65 | SP/HSF1-His-IF | TCAATTCGGGGGATCCATGAATAATGCTGCAAATACAGGGACGACC |
| 66 | ASP/HSF1-His-IF | TGCGGCCCTCCTGCAGCTAATGGTGATGGTGATGATGTTTCTTAGC |
| 82 | SP/HSF1del -KanMX | CCAAATAGCAGTACTGTTCCGGTAGATAAAGGCAAAGAGTTAGAGATGGGTAAGGAAAAGACTCA |
| 83 | ASP/HSF1del-KanMX | GAAAAACTCATCGAGCATCATTAAATGATTATATACGCTATTTAATGACCTTGCCCTGTGTACTA |
a Restriction sites are underlined.