TABLE 4.
Influence of TPS1 alleles on the viability of exponentially growing cells
Shown are results from flow cytometric analysis of viability of exponentially growing cells (A600 ∼1.0 unit) cultivated in YN synthetic medium supplemented with different carbon sources at 30 °C. The values are the means ± S.D. of five independent experiments. Gal Tre, 2% galactose + 1% trehalose; Gly Lac Eth, 3% glycerol + 2% lactate + 2% ethanol; ND, not determined; NG, no growth.
| Carbon source | CEN.PK113–7D background |
BY4741 background |
||||
|---|---|---|---|---|---|---|
| TPS1 | tps1Δ | tps1-156 | TPS1 | tps1Δ | tps1-156 | |
| Galactose 2% | 91 ± 4 | 63 ± 10 | 89 ± 4 | 86 ± 5 | 61 ± 12 | 83 ± 6 |
| Trehalose 2% | 84 ± 5 | 61 ± 8 | 86 ± 5 | NG | NG | NG |
| Gal Tre | 88 ± 6 | 66 ± 7 | 86 ± 4 | 81 ± 6 | 55 ± 8 | 84 ± 3 |
| Gly Lac Eth | 84 ± 5 | 59 ± 11 | 85 ± 7 | ND | ND | ND |