TABLE 5.

Intracellular trehalose quantification in the CEN.PK113–7D background strains

−Tre and +Tre indicate whether growth was performed in YN Gal or in YN GalTre medium, respectively. The values are the means ± S.D. of three biological replicates and are expressed in μg eq·glucose/10⁷ cells.

Condition	TPS1		tps1Δ		tps1−156
	−Tre	+Tre	−Tre	+Tre	−Tre	+Tre
2 h after upshift to the indicated temperature (unless otherwise stated in parentheses)
Control (30 °C; T0)	0	28 ± 8	0	20 ± 7	0	19 ± 6
37 °C (1 h)	0	46 ± 6	0	50 ± 7	0	49 ± 7
37 °C	0	40 ± 7	0	45 ± 5	0	42 ± 8
42 °C (1 h)	35 ± 6	45 ± 6	0	47 ± 8	0	41 ± 9
42 °C	22 ± 5	33 ± 7	0	51 ± 10	0	46 ± 9
45 °C	13 ± 4	36 ± 7	0	25 ± 9	0	32 ± 7
48 °C	3 ± 2	39 ± 9	0	33 ± 7	0	32 ± 6
50 °C	0	22 ± 8	0	8 ± 7	0	15 ± 6
52 °C	0	25 ± 6	0	18 ± 6	0	17 ± 3
2 h after temperature upshift to 50 °C, following a preheat for 1 h at the given temperature
Preheat at 37 °C	0	35 ± 8	0	41 ± 12	0	35 ± 11
Preheat at 42 °C	3 ± 2	21 ± 8	0	26 ± 9	0	31 ± 5
Under peroxide, salt or ethanol exposure for the indicated time
Control (30 °C; T0)	0	25 ± 7	0	23 ± 11	0	27 ± 8
50 mm H2O2 for 2 h	0	23 ± 6	0	9 ± 6	0	26 ± 9
1 m NaCl for 2 h	0	26 ± 5	0	19 ± 4	0	23 ± 5
5% EtOH for 6 h	8 ± 3	9 ± 6	0	4 ± 3	0	7 ± 5