FIGURE 3.

Phlpp1 deficiency enhances matrix production and Mek/Erk activation in chondrocytes. Chondrocyte micromass cultures from WT or Phlpp1^−/− mice were cultured for 3 days. A, cultures were fixed and stained with Alcian blue. Relative expression levels of type 2a1 collagen (B) aggrecan (C), PTHR (D), PRTrP (E), and Ihh (F) were assayed by real time PCR. *, p < 0.05 compared with control. G, Western blotting was performed as indicated. This experiment was repeated three times with data from a representative experiment shown.