FIGURE 3.

N- and C-terminal deletions activate the plant PM H⁺-ATPase in a yeast growth assay. A yeast strain with the native PM H⁺-ATPase placed under a galactose-inducible promoter was transformed with plasmids bearing wild-type or mutant plant PM H⁺-ATPases under the native promoter. Growth was assayed on minimal medium plates with either glucose or galactose at the given pH values and recorded after 3 days. Droplets contained initially around 10³ cells.