FIGURE 1.

Receptor-binding specificity of lymphotoxin-αβ heteromers of defined stoichiometries. A, schematic representation of “conventional” (FLAG LTα) and single-chain ligands (FLAG single-chain LTα-LTα-LTα, FLAG LT-ββα) in which individual monomers are covalently linked by a flexible linker of five amino acid residues. B, anti-FLAG Western blot (WB) of the indicated single-chain FLAG-tagged lymphotoxins. C, binding of the indicated FLAG-tagged conventional or single-chain ligands to immobilized TNFR1-Fc (upper panel) or LTβR-Fc (lower panel), as measured in an ELISA-based assay. LTα/Fc-LTβ is FLAG-LTα co-expressed with Fc-LTβ.