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SDS-PAGE analysis of single-chain BAFF-APRIL heteromers. A, Western blot analysis with 200 ng/lane of FLAG-tagged or 100 ng/lane of Fc-tagged single-chain BAFF-APRIL heteromers (except for Fc-AAA, ∼20 ng) revealed as indicated with either anti-BAFF Buffy-2, anti-FLAG M2, anti-APRIL Aprily-2 monoclonal antibodies, or with an anti-Fc antibody. B, Coomassie Blue staining of 6 μg/lane (except for AAB, 1 μg) of the indicated untagged single-chain BAFF-APRIL heteromers. WB, Western blot.
