ps193 |
FLAG-hTNF |
HA signal-FLAG-GPGQVQLQ-hTNF (aa 85–263) |
PCR3 |
ps420 |
hTNFR1-Fc |
hTNFR1 (aa 1–217)-VD-hIgG1 (aa 245–470) |
PCR3 |
ps515 |
EGFP |
Enhanced green fluorescent protein |
PCR3 |
ps645 |
FLAG-hLTa |
HA signal-FLAG-GPGQVQLQVD-hTNF (aa 43–205) |
PCR3 |
ps739 |
hBCMA-Fc |
Ig signal-EVKLVPRGS-hBCMA (aa 2–54)-VD-hIgG1 (aa 245–470) |
PCR3 |
ps897 |
hTACI-GPI |
HA signal-LD-hTACI (aa 2–160)-VD-hTRAILR3 (aa 157–269) |
PCR3 |
ps1196 |
Fc-hBAFF |
HA signal-LD-hIgG1 (aa 245–470)-RS-CamLinker-PreSci-GSLQ-hBAFF (aa 137–285) |
PCR3 |
ps1377 |
pMSCV-puro |
Modified pMSCV-puro (<zco;clontech>Clontech) with HindIII-BglII-EcoRI-NotI-XhoI-HpaI-ApaI cloning sites |
ps1377 |
ps1406 |
Fc-hLTb |
HA signal-LD-hIgG1 (aa 245–470)-RS-CamLinker-PreSci-GSLQ-hLTb (aa 66–244) |
PCR3 |
ps1467 |
hBCMA-GPI |
Ig signal-VQCEVKLVPRGS-hBCMA (aa 2–54)-VD-hTRAILR3 (aa 157–269) |
PCR3 |
ps1589 |
hBAFFR-GPI |
HA signal-LE-hBAFFR (aa 2–71)-EFGSVD-hTRAILR3 (aa 157–269) |
PCR3 |
ps1799 |
mLTbR-Fc |
mLTbR (aa 1–220)-VD-PreSci-hIgG1 (aa 245–470) |
PCR3 |
ps2297 |
mBAFFR-Fc |
HA signal-LD-mBAFFR (aa 2–70)-VD-hIgG1 (aa 245–470) |
PCR3 |
ps2308 |
hBAFFR:Fas |
HA signal-LE-hBAFFR (aa 2–71)-EFGSVD-hFas (aa 169–355) |
ps1377 |
ps2309 |
hBCMA:Fas |
Ig signal-VQCEVKLVPRGS-hBCMA (aa 2–54)-VD-hFas (aa 169–335) |
ps1377 |
ps2455 |
hTACI:Fas |
HA signal-L-hTACI (aa 1–118)-VD-hFas (aa 169–335) |
ps1377 |
ps2642 |
FLAG-hLT-ααα |
HA signal-FLAG-GPGQVQLQVD-hLTa (aa 58–205)-GGGGS-hLTa (aa 58–205)-GGGGS-hLTa (aa 58–205) |
PCR3 |
ps2643 |
FLAG-hLT-ααβ |
HA signal-FLAG-GPGQVQLQVD-hLTa (aa 58–205)-GGGGS-hLTa (aa 58–205)-GGGGS-hLTb (aa 83–244) |
PCR3 |
ps2644 |
FLAG-hLT-ββα |
HA signal-FLAG-GPGQVQLQ-hLTb (aa 83–244)-GGGGS-hLTa hLTb (aa 83–244)-GGGGS-hLTa (aa 58–205) |
PCR3 |
ps2645 |
FLAG-hLT-βββ |
HA signal-FLAG-GPGQVQLQ-hLTb (aa 83–244)-GGGGS-hLTa hLTb (aa 83–244)-GGGGS-hLTb (aa 83–244) |
PCR3 |
ps2680 |
Fc-BBB |
HA signal-LD-hIgG1 (aa 245–470)-RS-CamLinker-LQ-hBAFF (aa 140–285)-GGGGS-hBAFF (aa 140–285)-GGGGS-hBAFF (aa 140–285) |
PCR3 |
ps2826 |
Fc-hAPRIL |
HA signal-LD-hIgG1 (aa 245–470)-RS-CamLinker-GSLQ-hAPRIL (aa 115–250) |
PCR3 |
ps2876 |
Fc-AAA |
HA signal-LD-hIgG1 (aa 245–470)-RS-CamLinker-LQ-hAPRIL (aa 112–250)-GGGGS-hAPRIL (aa 112–250)-GGGGS-hAPRIL (aa 112–250) IRES GFP |
PCR3 |
ps2877 |
Fc-BAA |
HA signal-LD-hIgG1 (aa 245–470)-RS-CamLinker-LQ-hBAFF (aa 140–285)-GGGGS-hAPRIL (aa 112–250)-GGGGS-hAPRIL (aa 112–250) IRES GFP |
PCR3 |
ps2879 |
FLAG-AAA |
HA signal-FLAG-GPGQVQLQ-hAPRIL (aa 112–250)-GGGGS-hAPRIL (aa 112–250)-GGGGS-hAPRIL (aa 112–250) IRES GFP |
PCR3 |
ps2883 |
Fc-ABB |
HA signal-LD-hIgG1 (aa 245–470)-RS-CamLinker-LQ-hAPRIL (aa 112–250)-GGGGS-hBAFF (aa 140–285)-GGGGS-hBAFF (aa 140–285) IRES GFP |
PCR3 |
ps2886 |
FLAG-ABB |
HA signal-FLAG-GPGQVQLQ-hAPRIL (aa 112–250)-GGGGS-hBAFF (aa 140–285)-GGGGS-hBAFF (aa 140–285) IRES GFP |
PCR3 |
ps2889 |
FLAG-BAA |
HA signal-FLAG-GPGQVQLQ-hBAFF (aa 140–285)-GGGGS-hAPRIL (aa 112–250)-GGGGS-hAPRIL (aa 112–250) IRES GFP |
PCR3 |
ps2890 |
FLAG-BBB |
HA signal-FLAG-GPGQVQLQ-hBAFF (aa 140–285)-GGGGS-hBAFF (aa 140–285)-GGGGS-hBAFF (aa 140–285) IRES GFP |
PCR3 |
ps3413 |
hTACI-Fc |
Modified Ig signal (METDTLLLWVLLLWVPGVHG)-hTACI (aa 31–110) hIgG1 (aa 245–470; L258E, A353S, P354S) |
PCR3 |
pcDNA3.1_AAB |
AAB |
Ig signal-His6-FLAG-TEV-GS-hAPRIL (aa 111–250; T126A)-GGGGS-hAPRIL (aa 111–250; T126A)-GGGGS-hBAFF (aa 140–285) |
pcDNA3.1 |
pcDNA3.1_ABA |
ABA |
Ig signal-His6-FLAG-TEV-GS-hAPRIL (aa 111–250; T126A)-GGGGS-hBAFF (aa 140–285)-GGGGS-hAPRIL (aa 111–250; T126A) |
pcDNA3.1 |
pcDNA3.1_BAA |
BAA |
Ig signal-His6-FLAG-TEV-GS-hBAFF (aa 140–285)-GGGGS-hAPRIL (aa 111–250; T126A)-GGGGS-hAPRIL (aa 111–250; T126A) |
pcDNA3.1 |
pcDNA3.1_BBA |
BBA |
Ig signal-His6-FLAG-TEV-GS-hBAFF (aa 140–285)-GGGGS-hBAFF (aa 140–285)-GGGGS-hAPRIL (aa 111–250; T126A) |
pcDNA3.1 |
pcDNA3.1_BAB |
BAB |
Ig signal-His6-FLAG-TEV-GS-hBAFF (aa 140–285)-GGGGS-hAPRIL (aa 111–250; T126A)-GGGGS-hBAFF (aa 140–285) |
pcDNA3.1 |
pcDNA3.1_ABB |
ABB |
Ig signal-His6-FLAG-TEV-GS-hAPRIL (aa 111–250; T126A)-GGGGS-hBAFF (aa 140–285)-GGGGS-hBAFF (aa 140–285) |
pcDNA3.1 |