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. 2015 May 13;290(26):16383–16392. doi: 10.1074/jbc.M115.650127

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — SAXS of the phytochrome constructs. A, scattering of the phytochrome fragments (S) as a function of scattering angle (q) is plotted on a logarithmic scale. Dark (Pr) state samples are plotted as black lines; illuminated (mostly Pfr) state samples are plotted in gray lines. The scattering data of PAS-GAF-PHY is from Takala et al. (11). B, structural parameters calculated from the SAXS scattering. R_g, radius of gyration; D_max, maximum particle dimension; V_Porod, Porod volume; I₀, forward scattering. The molecular weights (MW_exp) were estimated from the I₀ of BSA using the formula MW_exp ≈ MW(BSA) × [I₀(sample)/I₀(BSA)], where MW(BSA) = 66 kDa. Theoretical molecular weights (MW_calc) were calculated from the protein sequence (58).