FIGURE 11.

R-CRT dimerization interferes with proteasomal degradation. CRT^−/− cells were transfected with WT-CRT-EGFP (A and C) or C146A-CRT-EGFP (B and D) and incubated in the absence (A and B) or presence (C and D) of MG132 for 2 h. Scale bar, 10 μm. Cells were analyzed as in Fig. 2. E, quantification of fluorescence intensity. Corrected total cell fluorescence is expressed as -fold change value relative to control (0 h). Data are shown as the mean ± S.E. ***, p < 0.001 for intragroup comparison; i.e. black bar versus white bar within CRT-EGFP or C146A-CRT-EGFP group. ###, p < 0.001 for intergroup comparison; i.e. white or black bar of C146A-CRT-EGFP group versus the corresponding bar of CRT-EGFP group. Results are representative of 150 cells from at least three separate experiments for each condition.