FIGURE 2.

R-CRT accumulation in the presence of lactacystin. A, NIH 3T3 cells were incubated with 10 μm lactacystin (LACTA) for 1 or 2 h (CONTROL: 0 h). Cells were analyzed by immunofluorescence using anti-R-CRT pAb. B, quantification of fluorescence intensity in immunofluorescence data (A). Corrected total cell fluorescence was expressed as -fold change value relative to control. Results shown are representative of 200 cells from at least three separate experiments for each condition. Scale bar, 10 μm. Data are shown as the mean ± S.E. *, p < 0.05; ***, p < 0.001 for comparison with control.