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Cytoplasmic expression of CRT. CHO cells transfected with Ub-CRT-EGFP were incubated with MG132 (10 μm) for 18 h. Cell lysates were immunoprecipitated (IP) with anti-GFP Ab followed by immunoblotting (WB) with anti-GFP. Polypeptides with sizes corresponding to the cleaved product and uncleaved precursor are indicated. A band corresponding to the Ub-CRT-EGFP precursor was detected only in the treated cells after prolonged exposure of the blots.
