FIGURE 6.

Viability and apoptosis control upon MG132 treatment. A, viability of cells expressing R-CRT-EGFP, CRT-EGFP, or E1V-CRT-EGFP, assessed by the reduction of resazurin at various intervals (12, 18, 21, 24 h) of treatment with 10 μm MG-132. Data are the means ± S.D. (n = 6). All comparisons were not significant at p > 0.05. B, flow cytometry histograms of cells stably expressing CRT-EGFP or R-CRT-EGFP stained with propidium iodide at various intervals (12, 18, 24 h) of treatment with 10 μm MG132. Values of 100% correspond to 50,000 cells. The data shown are the means of three independent experiments. MG132 treatment for 24 h with a dose of 10 μm did not induce apoptosis. The slight induction of apoptosis in R-CRT-EGFP cells at 24 h of MG132 treatment may have been due to accumulation of pro-apoptotic R-CRT in the plasma membrane (11).