FIGURE 8.

Arginylation-independent proteasomal degradation of CRT. Ate1^−/− cells were transfected with Ub-CRT-EGFP or Ub-R-CRT-EGFP and treated with MG132 for 18 h to cause accumulation of CRT-EGFP or R-CRT-EGFP. MG132 was removed, and cells were incubated in the absence or presence of CHX or CHX+MG132 for 2 h. A, immunoblotting. B, intensities of EGFP bands relative to β-actin were measured and plotted as a percentage of initial band intensity (control: 0 h). Data are shown as the mean ± S.E. of three independent experiments. *, p < 0.05.