FIGURE 9.

EGFP does not contribute to proteasomal degradation of CRT fusion proteins. Ate1^−/− cells were transfected with Ub-CRT-FLAG or Ub-R-CRT- FLAG and treated with MG132 for 12 h to cause accumulation of CRT-FLAG or R-CRT-FLAG. MG132 was removed, cells were incubated in the absence or presence of CHX (A) or CHX+MG132 (B) for 1 or 2 h, and proteins were separated and immunoblotted. C, intensities of FLAG bands relative to β-actin were measured and plotted as a percentage of initial band intensity (control: 0 h). Data are shown as the mean ± S.E. of three independent experiments. *, p < 0.05; **, p < 0.01.