Extended Data Figure 7.

Related to Figure 4

a, Schematic overview of C-terminal and N-terminal deletion mutants of MAD2L2. b, c, Expression analysis in U2OS cells of GFP-tagged WT MAD2L2 and C- and N-terminal MAD2L2 deletion mutants (b) and of FLAG-tagged WT MAD2L2 and MAD2L2-C70R and −L186A (c). d, Analysis of DDR foci formation of GFP-tagged WT MAD2L2 and C- and N-terminal MAD2L2 deletion mutants by IF detection of GFP and γH2AX in U2OS at 3 h after IR (n=2 for N-terminals, n=3 for C-terminals, ± s.e.m.). e, Analysis of WT, C70R and L186A MAD2L2 accumulation into DDR foci by IF detection of MAD2L2 and 53BP1 in U2OS at 3 h post IR (n=2, ± s.e.m.). f, g, Expression analysis in TRF2ts MEFs of GFP-tagged MAD2L2 and C- and N-terminal MAD2L2 deletion mutants (f) and of FLAG-tagged WT MAD2L2 and MAD2L2-C70R, −L186A, −T103A and −T103D (g). h, Quantification of chromosome fusions after 24 h of telomere deprotection at 39°C in TRF2ts MEFs transduced with control or Mad2l2 shRNAs and complemented with empty vector control or RNAi-resistant GFP-tagged WT MAD2L2 and C- or N-terminal MAD2L2 deletion mutants (Error bars: s.e.m.). i, Quantification of survival assays of TRF2ts MEFs transduced with control or Mad2l2 shRNAs and subsequently with empty vector control, WT MAD2L2, MAD2L2 C70R, L186A, T103A or T103D retroviruses (n=2, ± s.e.m.).