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. Author manuscript; available in PMC: 2015 Nov 28.
Published in final edited form as: Nature. 2015 Mar 23;521(7553):537–540. doi: 10.1038/nature14216

Extended Data Figure 4.

Extended Data Figure 4

Related to Figure 3

a, Representative images of IF detection of p-ATM(S1981), γ-H2AX, 53BP1 and RIF1 in TRF2ts MEFs transduced with control or Mad2l2 shRNAs and grown at 32°C or for 12 h at 39°C to induce telomere uncapping. DNA was stained with DAPI. b, Quantification of the number of p-ATM, γ-H2AX, 53BP1 and RIF1 foci per cell in TRF2ts MEFs transduced with control or Mad2l2 shRNAs and grown at 32°C or for 3 and 12 h at 39°C (n=2, ± s.d.). c, Quantifications of 53BP1, RIF1 and PTIP foci in U2OS cells transduced with control or MAD2L2 shRNAs, 3 h after 5Gy (n=2, ± s.d.).