Table 1.
Characteristic of the genes belonging to Fur regulon of L. monocytogenes
| Gene | Function/putative function of encoded proteina | Degree of Fur/ Fe controlb | Match and localisation of Fur site consensusd |
|---|---|---|---|
|
lmo0361 (tatC) |
Sec-independent protein secretion pathway, twin arginine translocase component C (TatC) | 22.75/3.61 | NA |
|
lmo0362 (tatA) |
Sec-independent protein secretion pathway, twin arginine translocase component A (TatA) | 19.75/4.29 |
GATAATGATAATCATTtTC −25 |
|
lmo0365 (fepC) |
Putative FTR1 family high-affinity Fe2+/Pb2+ permease (FepC) | 25.03/5.58 |
GATAATGATAATCATTtTC −26 |
|
lmo0366 (fepA) |
Putative lipoprotein involved in iron transport (FepA) | 27.61/5.47 | NA |
|
lmo0367 (fepB) |
Putative Dyp-type peroxidase (FepB) | 25.83/4.07 | NA |
| lmo0484 | Haem-degrading monooxygenase IsdG | 5.03/– |
GAcAtTGAgAATCATTATC −63 |
| lmo0485 | Hypothetical protein/putative nitroreductase from NADH oxidase and arsenite oxidase family | 3.83/2.18 |
GATAAcGtTTATCATTtaa −14 |
| lmo0541 | ABC transporter substrate-binding protein/putative ABC-type Fe3+-hydroxamate transport system | 7.01/2.62 |
GATAATGAaAATCATTtTC −21 |
|
lmo064 (frvA) |
Heavy metal-transporting ATPase—haem exporter FrvA | −5.64c/– |
GgTAATGggAATCATTATC −21 |
| lmo0642 | Hypothetical protein with unknown putative function | –/– | NA |
|
lmo0943 (fri or frm or frl) |
Non-haem iron-binding ferritin, DPS protein | 2.4/2.35 |
atTAAgGATAATCATTATa −20 |
| lmo1007 | Hypothetical protein with unknown putative function | 5.61/2.48 |
gATAATGATAATCATTtTC −42 |
| lmo1131 | ABC transporter ATP-binding protein/putative CydD-like transport system involved in cytochrome bd biosynthesis | 9.93/5.97 |
GAcAATGAgAATCATTATC −159 |
| lmo1132 | ABC transporter ATP-binding protein/putative MdlB-like multidrug transport system | –/– | NA |
|
lmo1956 (fur) |
Ferric uptake regulator Fur | NA/−1.44 |
GtaAtTGATAATCATTgTa −193 GATAATGATgATaATTtag −39 |
|
lmo1957 (fhuG) |
Ferrichrome ABC transporter permease FhuG | 5.86/2.22 | NA |
|
lmo1958 (fhuB) |
Ferrichrome ABC transporter permease FhuB | 4.7/2.01 |
GcgAtTGATAATtATTATC −44 |
|
lmo1959 (fhuD) |
Ferrichrome-binding protein FhuD | –/– |
GAgAATtATTATCAgTtaC −14 |
|
lmo1960 (fhuC) |
Ferrichrome ABC transporter ATP-binding protein FhuC | 2.44/2.02 |
GAgAATGATTATCAcctTa −23 |
| lmo1961 | oxidoreductase | 3.58/2.4 | NA |
|
lmo2104 (feoA) |
Ferrous iron transport system protein A, FeoA | 15.17/7 |
GATAATGATTATCATgtTC −33 |
|
lmo2105 (feoB) |
Ferrous iron transport system protein B, FeoB | 7.76/4.34 | NA |
| lmo2132 | Hypothetical protein/ putative regulatory protein Crp-like | 2.04/2 |
ttTAgTGATTATCgcTATa −136 |
|
lmo2429 (hupC) |
Haem ABC transporter ATP-binding protein HupC | 3.03/– | NA |
|
lmo2430 (hupG) |
Haem ABC transporter permease HupG | 3.12/– | NA |
|
lmo2431 (hupD) |
Haem ABC transporter substrate-binding protein HupD | –/– |
GAaAAaGATTATCAgTcat −156 GAaAATaATTcTCAaTtag −70 |
| lmo2180 | Hypothetical protein/putative siphovirus Gp157 protein | 4.09/4.71 | NA |
|
lmo2181 (srtB) |
Sortase B, SrtB | 12.83/4.6 | NA |
| lmo2182 | ABC transporter ATP-binding protein /putative ATP-binding component of iron-siderophores, vitamin B12 and hemin transporters and related proteins | 13.3/3.97 | NA |
| lmo2183 | ABC transporter permease/putative permease involved in the uptake of siderophores, haem or vitamin B12 | 15.76/4.48 | NA |
| lmo2184 | ABC transporter substrate-binding protein | –/– | NA |
|
lmo2185 (hbp2) |
Hemoglobin binding protein 2, Hbp2 | 8.91/2.99 | NA |
|
lmo2186 (hbp1 or svpA) |
Haemoglobin binding protein 1, Hbp1 | 9.48/4.24 |
GAcAATGATAATCATTATC −108 |
NA, not applicable; ‘–’, no change was observed or data not available
aPutative function of the gene is based on annotations provided by NCBI (http://www.ncbi.nlm.nih.gov/gene)
bLevel of the control is given according to Ledala et al. (2010); Fur control is given as ratios of expression levels in the fur mutant (Δfur) in the presence of iron (+Fe) to expression levels in the L. monocytogenes wild type (WT) in the presence of iron (Δfur + Fe/WT + Fe) whereas Fe control is given as ratios of expression levels in the L. monocytogenes wild type in iron-limiting conditions (−Fe) to expression levels in the L. monocytogenes wild type in the presence of iron (WT-Fe/WT + Fe)
cThe different result was obtained by McLaughlin et al. (2012)
dMatch is given in comparison to the 19 bp Fur-site consensus (5′GATAATGAT(a/t)ATCATTATC3′) of L. monocytogenes defined by McLaughlin et al. (2012), positive matches are in bold and capitalized letters whereas differences in consensus sequence are designated with small letters; localisation is given in relation to translation start site of the gene/operon