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. 2015 Jun 25;10(6):e0130808. doi: 10.1371/journal.pone.0130808

Fig 2. CXCR4+ small cells are required for repopulation of all subsets of 60As6 cells.

Fig 2

(A) Both CXCR4+ and CXCR4- 60As6 cells were sorted by FACS (left column). The cells of each fraction were cultured for 7 days and then analyzed for the expression levels of CXCR4 (right column). (B) Immunocytochemistry for MUC5AC (green) of a colony derived from a single CXCR4+ small cell. Scale bars represent 50μm. (C) Cell-lineage assay with two different colors. Sorted single CXCR4+ small cells were stained by anti-MUC5AC antibody (red) and CellTracker Green (Invitrogen, green) and for tracing the viable cells for 7 days. Scale bars represent 50μm. (D) RT-PCR of LGR5 in normal gastric epithelial cells (left panel), CXCR4+ small cells and CXCR4- small cells (right panel). Laser microdissection separates the normal gastric mucosa into three regions: pit, neck, and gland.