Fig 4. The effects of combining As3+ and As4S4 on cellular ROS accumulation.
(A) Cellular ROS were determined with DCFH-DA fluorescence probe in NB4 cells. (B) Cellular ROS in primary APL cells. (C) The effects of As4S4 and As3+ on HMOX1 expression. (D) The percentage of relative HMOX1 intensity obtained by RT-PCR. Error bars represent the S.D. from the mean of three independent experiments. *P<0.05 compared with As3+ and As4S4 combination treated cells.