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. 2015 Jun 26;5:11596. doi: 10.1038/srep11596

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HaCat cells were treated with different concentration of ebselen ranging from 0 to 128 μg/ml. DMSO was used as a negative control. Cell viability was measured by MTS assay and IC₅₀ of ebselen to cause cytotoxicity in HaCat cells was calculated.