Fig 1. CRH down-regulates ABCA1 and LXR-α.

(A) Quantitative real-time reverse transcriptase PCR (qRT-PCR) was performed with RNAs from murine peritoneal macrophages treated with or without CRH (10 nM) for 18 hours. GAPDH was used as the internal control. Relative mRNA levels of indicated molecules were compared between untreated (black bar) and CRH-treated (white bar) macrophages. (B) qRT-PCR was performed using RNAs from human peripheral blood monocyte-derived macrophages treated with or without CRH (20 nM) for 18 hours. GAPDH was used as the internal control. Relative mRNA levels of indicated molecules were compared between untreated (black bar) and CRH-treated (white bar) macrophages. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.