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. 2015 Jun 25;10(6):e0130587. doi: 10.1371/journal.pone.0130587

Fig 2. CRH induces a decrease in macrophage ABCA1 expression.

Fig 2

(A) Western blot analysis for ABCA1 was performed using the cell lysates from murine peritoneal macrophages treated with or without CRH for 18 hours. (B) Western blot analysis for ABCA1 was performed using the cell lysates from murine peritoneal macrophages treated with or without CRH in the absence or presence of oxLDL (50 μg/ml). (C) Semi-quantitative reverse transcriptase-PCR for ABCA1. mRNA was extracted from macrophages treated as in (A). (D) Macrophages treated with or without CRH (10 nM) for 18 hours were lysed, and mRNA from these cells were utilized in semi-quantitative reverse transcriptase PCR for LXR-α. (E) Human monocyte-derived macrophages were treated with or without CRH (20 nM) in the absence or presence of oxLDL(50 μg/ml) for 18 hours. Western blot for ABCA1 was performed with the cell lysates. Quantitative data are presented as mean ± SEM (right graphs). * P < 0.05, ** P < 0.01, *** P < 0.001.