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Mild ambient temperatures below thermoneutrality induce FGF21 serum levels and secretion from BAT of UCP1 KO mice. Mice were maintained at 30 °C or exposed to mild cold (23 °C or 18 °C) (A) Serum levels of FGF21 and ex vivo secretion of FGF21 from (B) BAT, (C) iWAT, EDL (extensior digitorum longus) (EDL) and (F) soleus muscle of WT and UCP1 KO mice after 4 h incubation. *P < 0.05; ***P < 0.001, significant differences between the genotypes. Data are means ± SEM (n = 4–6/group).
