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. 2015 May 1;4(7):507–518. doi: 10.1016/j.molmet.2015.04.003

Figure 3.

Figure 3

Streptozotocin treatment reduces hyperinsulinemia and attenuates inflammation in AT from ob/ob mice. (A) Time line of streptozotocin administration. Five-week-old ob/ob mice were i.p. injected once a day for 5 days with the indicated dose of streptozotocin or control citrate buffer. Two weeks later, an additional single dose of streptozotocin (200 mg/kg) was i.p. injected. (B) Pancreata from 12-week-old mice were isolated, fixed, sectioned, and stained with anti-insulin antibody. Shown are islets and insulin-stained β-cells. Images are representative of mice in each group. (C) Insulin and c-peptide serum levels in WT, ob/ob and streptozotocin (STZ)-treated ob/ob mice. (D) Adipose or (F) liver tissues from WT and ob/ob mice treated or not with the indicated doses of streptozotocin were harvested and mRNAs measured by quantitative RT-PCR analysis. Depicted are the relative expression levels of indicated pro-inflammatory genes. (E) Representative immunoblot of CCL2 and TNFα proteins in AT lysates from 12-week-old WT or ob/ob mice treated with indicated doses of streptozotocin. (G) H&E staining of AT sections, with crown-like structures (indicated by arrows) from WT and control or STZ-treated ob/ob mice. Graphs show the mean ± SEM; N = 5–6 per group, compared with chow controls, by Student's t test *P < 0.05; **P < 0.01; ****P < 0.0001.