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. 2015 Jun 25;11(6):e1005167. doi: 10.1371/journal.pgen.1005167

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© 2015 Burla et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) ChIPs from HPFs, uninfected HeLa cells and shAKTIP-11-infected HeLa cells reveal interactions between AKTIP and telomeric DNA. Chromatin was immunoprecipitated with an anti-AKTIP antibody or control IgGs; slot-blots were hybridized with TTAGGG or ALU repeat probes. (B) ChIP quantification after normalization to the input (levels shown in A). Bars show the mean values of two experiments ± SD; the amount of telomeric DNA precipitated from uninfected HeLa cells is significantly higher than that obtained from shAKTIP-11-infected cells (*p<0.05 in the Student t test). (C, D) AKTIP-GST pulls down TRF1 (C) and TRF2 (D) from 293T cell extracts. In, input; M, MW markers.