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. 2015 Mar 13;106(2):261–271. doi: 10.1093/cvr/cvv108

Figure 1.

Figure 1

ANKRD1 regulates GATA4 DNA-binding and activity. (A) NRVM transfected with a GATA4-dependent luciferase reporter (black bars) or control (reporter with no GATA4 binding sites; white bars) and treated with 100 µM PE in the presence or absence of Ankrd1 siRNA. Values normalized to untreated non-silencing siRNA (NS) control and expressed as mean ± SEM, n = 4–6, *P < 0.05 vs. untreated NS control. (B) Electrophoretic mobility shift assay using NRVM nuclear extracts revealed a single band shift with an α-MHC probe which was specific for GATA4 as shown by co-incubation with anti-GATA4 antibody resulting in a supershift (SS), or loss of band with cold or mutant (Mut) α-MHC probe. Co-incubation with anti-ANKRD1 antibody or using nuclear extracts from NRVM treated with Ankrd1 siRNA resulted in depletion of the GATA4 band. Asterisks indicate non-specific bands. Representative image from three independent experiments.