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. 2015 Feb 20;24(10):2873–2883. doi: 10.1093/hmg/ddv049

Figure 2.

Figure 2.

Trim32 is able to ubiquitinate recombinant NDRG2 and Trim72 proteins in vitro. (A) Trim32 ubiquitinated NDRG2 in conjunction with UbcH5a, UbcH5b and UbcH5C. Recombinant Trim32 was incubated in the presence of ATP, ubiquitin, E1 and several different E2 s as specified in the figure. Lane (–E2) indicates no E2 was added. The panel shows blot stained with anti-NDRG2 Ab. (B) Trim32 ubiquitinated Trim72 in conjunction with UbcH5b E2. The panel shows blot stained with anti-Trim72 Ab. Trim32 specifically ubiquitinated NDRG2 and TRim72 as evidenced by ∼10 kDa increment shifts in the molecular mass of the target proteins.