Figure 3.

Levels of NDRG2 protein are significantly reduced in ubiquitinated fractions from T32KO muscles. (A and B) Western blot analysis of levels of NDRG2 and Trim 72 proteins in ubiquitinated fractions eluted from ubiquitin-affinity resin. Total muscle lysates from T32KO and WT muscles were incubated with ubiquitin-affinity resin (anti-ubiquitin monoclonal antibody (clone FK2) covalently coupled to agarose gel, ‘MBL’) for 2 h at RT, extensively washed, and bound proteins were eluted by reducing sample buffer. Panel A shows blot stained with anti-NDRG2 Ab, and panel B shows blot stained with anti-Trim72 Ab. Samples from four animals per group were analyzed and representative blots are shown. The Ponceau S stained blots are shown as a loading control. Densitometry data normalized to loading, data are shown as mean ± SEM (*P < 0.05). Statistical analysis by t-test.