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. 2015 Feb 20;24(10):2873–2883. doi: 10.1093/hmg/ddv049

Figure 5.

Figure 5.

NDRG2 accumulates in WT myoblasts in the presence of proteasome inhibitor MG132. (A and B) Primary myogenic cells were treated with 50 μm of the inhibitor of proteasome activity, MG132, for 1, 3 or 6 h. Cells represented by the lane labeled ‘0’ were treated with dimethyl sulfoxide (vehicle) alone for 6 h. NDRG2 accumulated in WT myoblasts in the presence of MG132; in the T32KO cells the levels of NDRG2 were maintained, independent of the presence of MG13. Anti-vinculin blots and Ponceau S stained blots are shown as a loading control. Densitometry data normalized to loading and shown as mean ± SEM. Statistical analysis by t-test.