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. 2015 Feb 20;24(10):2873–2883. doi: 10.1093/hmg/ddv049

Figure 6.

Figure 6.

Over-expression of NDRG2 causes a delay in cell cycle withdrawal and slows down cell proliferation. (A and B) Cell cycle analysis of C2C12 myoblasts over-expressing NDRG2. C2C12 myoblasts were stained with PI and analyzed by flow cytometry. (A, B) Representative flow cytometric histograms showing the cell cycle distribution. (A) C2C12 control- and NDRG2-transduced cells in GM. (B) C2C12 control- and NDRG2-transduced cells were cultured for 18 h in DM. G1/G0, S and G2/M peaks are shown. Percentages of dividing (div) (S+G2/M) and non-dividing (nondiv.) (G1/G0) cells were calculated. Values represent the mean of at least three independent experiments ± SEM. (C) Effect of elevated NDRG2 levels on C2C12 myoblast proliferation. Cell proliferation index is presented as a fold change of the number of cells in 24 h. Data are summarized and presented as the mean of three independent experiments ± SEM (*P < 0.05). Statistical analysis by t-test. (D) Positions of G1 and G2 peaks are not changed on the PI fluorescence axis in NDRG2-transduced cells. Data are summarized and presented as the mean of three independent experiments ± SEM.