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. 2015 Jun 26;6:649. doi: 10.3389/fmicb.2015.00649

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Copyright © 2015 Merino, Aquilini, Fulton, Twine and Tomás.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plesiomonas shigelloides E mutant (as an example for the insertional lateral flagella mutants). TEM of the E mutant grown in liquid medium (A) and swarming agar plates (B) and complemented mutant with COS-LAFI harboring the corresponding wild type gene grown in semisolid medium (C). As could be observed in B the polar flagella are constitutively expressed in semisolid medium. Bar, correspond to 0.5 μm. Motility of the E mutant in swimming (D) and swarming (E) agar plates. The complemented mutant with COS-FLAregI-1harbouring the corresponding wild type gene in swarming agar plate (F).