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. 2015 Jun 26;6:649. doi: 10.3389/fmicb.2015.00649

FIGURE 6.

FIGURE 6

(A) Plesiomonas shigelloides 302-73 wild type strain serotype O1 purified flagella according to Section “Materials and Methods” when grown in liquid medium (1) and swarming agar plates (2). As could be observed in 2, and previously indicated in Figure 3, polar flagella are constitutively expressed in semisolid medium. Purified flagella from P. shigelloides insertional polar A mutant grown in swarming agar plates (3). (B). Western blot with specific polar flagella antiserum of purified flagella from wild type (1), P. shigelloides insertional polar H mutant (2), and complemented mutant with COS-LEG harboring the corresponding wild type gene (3) obtained in liquid medium growth. (C) Western blot with specific lateral flagella antiserum of purified flagella from wild type (1), P. shigelloides insertional polar H mutant (2), and complemented mutant with COS-LEG harboring the corresponding wild type gene (3) obtained in swarming agar plates. (D) Western blot with specific polar flagella antiserum of cytoplasmic fractions obtained as described in Section “Materials and Methods” of wild type (1), P. shigelloides insertional polar H mutant (2), and complemented mutant with COS-LEG harboring the corresponding wild type gene (3) obtained in liquid medium growth. The low molecular weight band could correspond to the non-glycosylated form, and the upper band (not present in the mutant) to the glycosylated form.