Table 1.
Bacterial strains and plasmids used.
| Strain or plasmid | Relevant characteristicsa/ | Source or reference |
|---|---|---|
| Escherichia coli | ||
| DH5α | F- endA hsdR17 (rk- mk+) supE44 thi-1 recA1 gyr-A96 80lacZ | Hanahan (1983) |
| S17-1λpirKm1 | thi thr1 leu tonA lacY supE recA::RP4-2 (Tc::Mu)Kmr λpir with miniTn5Km1 | De Lorenzo et al. (1990) |
| MC1061λpir | thi thr1 leu6 proA2 his4 argE2 lacY1 galK2 ara14 xyl5 supE44 λ pir | Rubirés et al. (1997) |
| Plesiomonas Shigelloides | ||
| 302-73 | Wild type, serotype O12:K80 | Pieretti et al. (2010) |
| 302-73R | 302-73, spontaneous Rifr | Aquilini et al. (2013) |
| A | 302-73flgE:mini-Tn5Km1 Rifr Kmr | This study |
| B | 302-73flhA:mini-Tn5Km1 Rifr Kmr | This study |
| C | 302-73fliI:mini-Tn5Km1 Rifr Kmr | This study |
| D | 302-73flgK:mini-Tn5Km1 Rifr Kmr | This study |
| E | 302-73lafA:mini-Tn5Km1 Rifr Kmr | This study |
| F | 302-73flhAL:mini-Tn5Km1 Rifr Kmr | This study |
| G | 302-73flgEL:mini-Tn5Km1 Rifr Kmr | This study |
| H | 302-73ptmA:mini-Tn5Km1 Rifr Kmr | This study |
| I | 302-73legH:mini-Tn5Km1 Rifr Kmr | This study |
| Plasmids | ||
| pLA2917 | Tcr, Kmr | Allen and Hanson (1985) |
| COS-FLAregI-1 | pLA2917 with 20-kb chromosomal 302-73 Sau3A insert carrying part of the polar flagella biosynthesis region I,Tcr | This study |
| COS-LAFI | pLA2917 with 20-kb chromosomal 302-73 Sau3A insert carrying part of the lateral flagella biosynthesis region, Tcr | This study |
| COS-LEG | pLA2917 with 20-kb chromosomal 302-73 Sau3A insert carrying complete Leg biosynthesis region, Tcr | This study |
| pRK2073 | Helper plasmid, Spr | Canals et al. (2006) |
| pGEM-T | PCR cloning vector, Ampr | Promega |
| pDM4 | pir dependent with sacAB genes, oriR6K, CmR | Milton et al. (1996) |
| pDM4ΔpgmL | pDM4 with truncated in frame pgmL | This study |
| pDM4ΔlegF | pDM4 with truncated in frame legF | This study |
| pBAD33 | Arabinose inducible expression vector, CmR | ATCC |
| pBAD33-pgmL | pBAD33 with complete pgmL | This study |
| pBAD33-legF | pBAD33 with complete legF | This study |
a/ = resistant.