Figure 1.

(A) Representative immunoblot showing protein carbonyl content before and after exposure to 100 μM H₂O₂ in six mammalian species with a maximum life span (MLS) range of 3.9–23.4 years. Protein carbonyls are labeled with 2,4-dinitrophenol, and protein carbonyl content is measured using an antibody against 2,4-dinitrophenol (anti-DNP). Total protein is measured using an India Ink stain. (B) Scatterplot of % change in protein carbonyl content following exposure to 100 μM H₂O₂ in 17 species of rodents.