Egg follicle data in Culex females. (A) Percent of Culex females categorized as being in a diapause, intermediate, or nondiapause state based on egg follicle length. All individuals were reared under diapause-inducing conditions, a photoperiod of 8:16 (L:D) h at 18°C. Two-week-old females were defined as being in a diapause state if mean length of 10 egg follicles was <75 µm, a nondiapause state if mean follicle length was >90 µm, and an intermediate state if mean follicle length was 76–89 µm. Cx. pipiens n = 15; female Cx. pipiens × male Cx. quinquefasciatus n = 148; female Cx. quinquefasciatus × male Cx. pipiens n = 178; Cx. quinquefasciatus n = 15. Bars represent SEs, and different letters indicate significant differences in diapause status between the two sets of hybrid mosquitoes. (B) Mean ± SE egg follicle length of 2-wk-old female mosquitoes reared under diapause-inducing conditions (a photoperiod of 8:16 [L:D] h; 18°C). Different letters indicate significant differences. Cx. pipiens n = 15; female Cx. pipiens × male Cx. quinquefasciatus n = 148; female Cx. quinquefasciatus ×male Cx. pipiens n = 178; Cx. quinquefasciatus n = 15. One-way ANOVA F = 20.47; df = 355; P < 0.0001.