Table 1. Synthesized sequences of N-terminus annexin isoforms and the sequences of CPP-PLP conjugates.
Name | Sequence | [M+H]+calca | [M+H]+obsb | pI | Chargec |
---|---|---|---|---|---|
AA1H | FITC-MAMVSEFLKQ | 1685.0 | 1685.6 | 5.61 | −1 |
AA2H | FITC-MSTVHEILCK | 1662.0 | 1662.6 | 8.86 | −1 |
AA3H | FITC-MASIWVGHRG | 1614.9 | 1615.8 | 10.06 | 0 |
AA4H | FITC-MATKGGTVKA | 1464.7 | 1464.9 | 10.80 | 1 |
AA5H | FITC-MAQVLRGTVT | 1576.9 | 1578.9 | 10.06 | 0 |
AA6H | FITC-MAKPAQGAKY | 1565.8 | 1564.2 | 10.23 | 1 |
AA7H | FITC-MSYPGYPPTG | 1570.8 | 1571.9 | 5.28 | −1 |
AA8H | FITC-MAWWKSWIEQ | 1866.2 | 1865.1 | 5.61 | −1 |
AA9H | FITC-MSVTGGKMAP | 1479.8 | 1478.9 | 8.86 | 0 |
AA10H | FITC-MFCGDYVQGT | 1621.8 | 1620.9 | 3.75 | −1 |
AA11H | FITC-MSYPGYPPPP | 1606.8 | 1605.4 | 5.28 | −1 |
AA13H | FITC-MGNRHAKASS | 1559.7 | 1560.4 | 11.66 | 1 |
TAT47-58 | FITC-YGRKKRRQRRR | 2061.4 | 2060.1 | 12.81 | 7 |
Sequences of protein conjugated CPPs | |||||
Name | Sequence | [M+H]+calc† | [M+H]+obs‡ | pI | Charge |
PLP | FITC-RRRQQQQQQRRR | 2225.5 | 2226.9 | 13.20 | 5 |
AA3H-PLP | FITC-MASIWVGHRG-RRRQQQQQQRRR | 3434.0 | 3433.8 | 13.28 | 7 |
TAT-PLP | FITC-YGRKKRRQRRR-RRRQQQQQQRRR | 3880.0 | 3878.6 | 13.74 | 13 |
aCalculated molecular weight.
bObserved molecular weight in MALDI-TOF MS.
cAt pH 7.4.