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. 2015 Jun 26;5:11719. doi: 10.1038/srep11719

Table 1. Synthesized sequences of N-terminus annexin isoforms and the sequences of CPP-PLP conjugates.

Name Sequence [M+H]+calca [M+H]+obsb pI Chargec
AA1H FITC-MAMVSEFLKQ 1685.0 1685.6 5.61 −1
AA2H FITC-MSTVHEILCK 1662.0 1662.6 8.86 −1
AA3H FITC-MASIWVGHRG 1614.9 1615.8 10.06 0
AA4H FITC-MATKGGTVKA 1464.7 1464.9 10.80 1
AA5H FITC-MAQVLRGTVT 1576.9 1578.9 10.06 0
AA6H FITC-MAKPAQGAKY 1565.8 1564.2 10.23 1
AA7H FITC-MSYPGYPPTG 1570.8 1571.9 5.28 −1
AA8H FITC-MAWWKSWIEQ 1866.2 1865.1 5.61 −1
AA9H FITC-MSVTGGKMAP 1479.8 1478.9 8.86 0
AA10H FITC-MFCGDYVQGT 1621.8 1620.9 3.75 −1
AA11H FITC-MSYPGYPPPP 1606.8 1605.4 5.28 −1
AA13H FITC-MGNRHAKASS 1559.7 1560.4 11.66 1
TAT47-58 FITC-YGRKKRRQRRR 2061.4 2060.1 12.81 7
           
Sequences of protein conjugated CPPs
Name Sequence [M+H]+calc [M+H]+obs pI Charge
PLP FITC-RRRQQQQQQRRR 2225.5 2226.9 13.20 5
AA3H-PLP FITC-MASIWVGHRG-RRRQQQQQQRRR 3434.0 3433.8 13.28 7
TAT-PLP FITC-YGRKKRRQRRR-RRRQQQQQQRRR 3880.0 3878.6 13.74 13

aCalculated molecular weight.

bObserved molecular weight in MALDI-TOF MS.

cAt pH 7.4.