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hK2-spiked human female serum analyzed with the sandwich microarray at two different capturing antibody concentrations (75 µg/mL and 100 µg/mL). The LOD was 10 pg/mL and 1 pg/mL when the concentrations of capture antibody were 75 µg/mL and 100 µg/mL, respectively. Increased assay sensitivity was observed with an elevated concentration of the capturing antibody (6B7). The negative signal also increased at the higher concentrations of the capture antibody.
