Table 2.
Excitation and emission wavelengths for some fluorophores used in biotechnology.
| Fluorophore | Max Excitation Wavelength (nm) | Max Emission Wavelength (nm) | Reference | |
|---|---|---|---|---|
| GFP | fluorescence proteins | 400, 470 | 505, 540 | [62] |
| EYFP | 514 | 527 | [62] | |
| mCherry | 587 | 610 | [65] | |
| Tryptophan | amino acids | 280, 290 | 350 | [63,66] |
| Tyrosine | 275/278 | 280, 300/330–350 | [63,66,67] | |
| Phenylalanine | 260 | 280, 282 | [63,68] | |
| FAD, Flavins | co-enzymes | 450 | 535 | [63] |
| NADH | 290, 351 | 440, 460 | [63] | |
| NAD(P)H | 336 | 464 | [63] | |
| Pyrodoxin | vitamins | 332, 340 | 400 | [63] |
| Vitamin A | 327 | 510 | [63] | |
| Riboflavin | 365 | 520 | [66] |