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. 2015 May 5;15(5):10481–10510. doi: 10.3390/s150510481

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Schematic representation of DNA-directed immobilization. (A) Conjugation of four covalent conjugates (HA-HD) with the biotinylated antibodies anti-carcinoembryonic antigen (RAC), anti-ceruloplasmin (SAC), anti-complement-1-inactivator (SCI), and anti-lectin (GAL) to generate capture reagents A-RAC, B-SAC, C-SCI, and D-GAL. Adapted from [97]; (B) Conjugation of Streptavidin-Cy5 with biotinylated RAC, SAC, SCI and GAL for the use of a microscaled fluorescence immunoassay. Adapted from [97]; (C) DNA-directed immobilization of DNA-linked antibodies. c-A (Target sequence conjugated to anti-hCG): 5'-AGC GGA TAA CAA TTT CAC ACA GGA-3'; c-B (Target sequence conjugated to anti-hLH): 5'-AAC AGC TAT GAC CAT GAT TAC-3'; OEG, oligo (ethylene glycol). Adapted from [98].