Table 2.
Primers used for PCR
| Primer | Sequence (5′–3′) | Target gene | Product (bp) | References |
|---|---|---|---|---|
| β-Lactamases | ||||
| TEM-F | CATTTCCGTGTCGCCCTTAT | bla TEM | 793 | [45] |
| TEM-R | TCCATAGTTGCCTGACTCCC | |||
| PSE-1-F | AATGGCAATCAGCGCTTCCC | bla PSE-1 | 586 | [46] |
| PSE-1-R | GGGGCTTGATGCTCACTACA | |||
| OXA-F | ACCAGATTCAACTTTCAA | bla OXA | 590 | [47] |
| OXA-R | TCTTGGCTTTTATGCTTG | |||
| SHV-F | TTATCTCCCTGTTAGCCACC | bla SHV | 795 | [48] |
| SHV-R | GATTTGCTGATTTCGCTCGG | |||
| CTX-M-F | ACCGCGATATCGTTGGT | bla CTX-M | 550 | [49] |
| CTX-M-R | TTAGTGACCAGAATCAGCGG | |||
| Plasmid-mediated quinolone resistance | ||||
| qnrA-F | ATTTCTCACGCCAGGATTTG | qnrA | 516 | [35] |
| qnrA-R | GATCGGCAAAGGTTAGGTCA | |||
| qnrB-F | GATCGTGAAAGCCAGAAAGG | qnrB | 476 | [35] |
| qnrB-R | ACGATGCCTGGTAGTTGTCC | |||
| qnrS-F | ACGACATTCGTCAACTGCAA | qnrS | 417 | [35] |
| qnrS-R | TAAATTGGCACCCTGTAGGC | |||
| aac(6′)-Ib-cr-F | CCCGCTTTCTCGTAGCA | aac(6′)-Ib-cr | 544 | [50] |
| aac(6′)-Ib-cr-R | TTAGGCATCACTGCGTCTTC | |||
| qepA-F | CGTGTTGCTGGAGTTCTTC | qepA | 403 | [51] |
| qepA-R | CTGCAGGTACTGCGTCATG | |||