Table 3.
Some examples of the differentially phosphorylated proteins identified in this study
| Peptide sequence | Protein group accessions | Annotation | Reported function | Ref | Modifications on peptide | Phos intensity 24 h/0 h |
|---|---|---|---|---|---|---|
| FLTASGTFKDGELR | LOC_Os01g32660.4 | STE_MEK_ste7_MAP2K.2 | Involved in cold stress signaling | [65, 66] | T7 (Phospho) | 24 h specific |
| NQHLLSPR | LOC_Os01g60700.1 | Serine/threonine-protein kinase NAK | S6 (Phospho) | 24 h specific | ||
| RSQEEDEVEER | LOC_Os02g50970.1 | Protein kinase domain containing protein | Mediates drought resistance through ROS scavenging | [67] | S2 (Phospho) | 0 h specific |
| SISAEGLHSLR | LOC_Os02g55560.1 | Protein phosphatase 2C, putative | S3 (Phospho) | 24 h specific | ||
| LTSVVEEDNRGEEVVEEEAR | LOC_Os03g18070.1 | Omega-3 fatty acid desaturase, chloroplast precursor | May be involved in heat tolerance | [68] | S3 (Phospho) | 0 h specific |
| MSPAEASREENVYMAK | LOC_Os03g50290.1 | 14-3-3 protein | Involved in Biotic and Abiotic Stress response | [69] | N-Term (Acetyl); S2 (Phospho) | 24 h specific |
| APSSAGAAAGRPGLMVLR | LOC_Os03g59390.1 | CAMK_CAMK_like.24 | S3 (Phospho) | 2.147117423 | ||
| LMDYKDTHVTTAVR | LOC_Os04g38480.1 | BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 precursor | Regulates rice leaf development | [70] | T7 (Phospho); T10 (Phospho) | 24 h specific |
| NAPGGPLSPGGFPMNRPGTGGMMPGMPGTPGMPGSR | LOC_Os04g42140.1 | Eukaryotic initiation factor iso-4 F subunit p82-34 | Confers high resistance of rice to Rice yellow mottle virus | [43, 71] | S8 (Phospho) | 0 h specific |
| ASGGGGEMGPVLQR | LOC_Os04g47300.1 | CAMK_CAMK_like.26 | Oppositely modulates salt-stress tolerance and blast disease resistance | [40] | S2 (Phospho) | 0.4237053 |
| HDTDDNNNAAAADSPKKPSRPPAAAK | LOC_Os04g49510.1 | CAMK_CAMK_like.27 | Confers both cold and salt/drought tolerance on rice | [72] | S14 (Phospho) | 0 h specific |
| EMSDDESTDKLLVEPQK | LOC_Os04g58620.1 | Potasium efflux antiporter protein | Regulates chloroplast development and drought resistance | [73] | S3 (Phospho) | 0.290186904 |
| ALNNIMHMSNSPTSSYR | LOC_Os05g03430.3 | ATSIZ1/SIZ1, E3 Ubiquitin ligase | Regulates Vegetative and reproductive Development, enhances broad abiotic stress tolerance | [74] | T13 (Phospho) | 0 h specific |
| SIHGSQLGTVTEAEHS | LOC_Os05g05590.1 | Transporter, monovalent cation:proton antiporter-2 family | Enhances rice sanity tolerance | [75] | S1 (Phospho) | 0.496889646 |
| KLVNSSFADLQKPQMELDGK | LOC_Os05g38150.1 | Amino acid synthetase | Enhances rice sanity and drought tolerance | [76] | S5 (Phospho) | 0 h specific |
| TINESMDELSSQSK | LOC_Os05g47560.1 | Serine/threonine-protein kinase SNT7, chloroplast precursor | T1 (Phospho); M6 (Oxidation) | 24 h specific | ||
| IAHIPKPEASLDSLSFK | LOC_Os05g50710.1 | Late embryogenesis abundant protein | Enhances the cell tolerance to various biotic and abiotic stresses | [77] | S15 (Phospho) | 24 h specific |
| VSQPAEEDEMDFDSEEVEDEEEEEK | LOC_Os05g51830.1 | ZOS5-12 - C2H2 zinc finger protein, Histone Deacetylase | Negatively Regulates Plant Innate Immunity | [61] | S14 (Phospho) | 0 h specific |
| TTSETDFMTEYVVTR | LOC_Os06g06090.2 | CGMC_MAPKCMGC_2_ERK.12 | Activates rice innate immunity | [41, 78] | T9 (Phospho); Y11 (Phospho) | 0.473270364 |
| LYEHGATPATTR | LOC_Os06g10790.1 | Lectin-like receptor kinase | T10 (Phospho) | 24 h specific | ||
| DFGSMNMDELLR | LOC_Os06g10880.3 | bZIP transcription factor | Responds to ABA and IAA | [79] | S4 (Phospho) | 24 h specific |
| QIDASDLPSDDSADNDYDPTLAQGHK | LOC_Os06g12400.1 | Homeobox domain containing protein | Regulates GA response | [80] | S5 (Phospho); S9 (Phospho) | 0 h specific |
| SISAEGLR | LOC_Os06g39600.2 | Protein phosphatase 2C, putative | S3 (Phospho) | 2.320807642 | ||
| DGGAASEYLIEEEEGLNEHNVVEK | LOC_Os06g43660.3 | Inorganic H+ pyrophosphatase | Enhances rice chill tolerance | [81] | S6 (Phospho) | 0 h specific |
| NVSPAEQSAADK | LOC_Os06g44210.1 | Protein phosphatase 2C, putative | S3 (Phospho) | 0 h specific | ||
| RPFPPPSPAK | LOC_Os06g50030.1 | CAMK_CAMK_like.30 | S7 (Phospho) | 3.145113364 | ||
| SFDELSDDEGLYEDSD | LOC_Os07g39870.2 | Eukaryotic peptide chain release factor subunit 1-1 | Involved in chill and drought stress | [82] | S6 (Phospho) | 0 h specific |
| ITVLTSDGSTARPKPIQK | LOC_Os08g01900.1 | E3 ubiqutin ligase | Reduced cellular oxidative stress | [48] | S9 (Phospho) | 0.419643669 |
| LNSFYISHNR | LOC_Os08g14950.1 | Receptor-like protein kinase 2 precursor | S3 (Phospho) | 24 h specific | ||
| AGAGAGASPGWPQR | LOC_Os08g39100.1 | Protein phosphatase 2C, putative | S8 (Phospho) | 0 h specific | ||
| AEELVGASPGTEGMSSAEAK | LOC_Os09g34060.1 | Transcription factor RF2a | Enhances rice resistance to rice tungro disease | [58, 59, 83] | S8 (Phospho) | 24 h specific |
| SSTPAAAAEQEHR | LOC_Os10g42430.1 | Transcription factor MYC7E | Involved in JA signaling | [44] | S1 (Phospho) | 24 h specific |
| SPHGGDGDGAAGDDGGDAQAAAAGGR | LOC_Os11g29870.1 | OsWRKY72 - Superfamily of TFs having WRKY and zinc finger domains | Involved in ABA response | [84] | S1 (Phospho) | 24 h specific |
| GSLGSLNMITGK | LOC_Os12g13170.3 | Transcription factor | Involved in ABA response | [85] | S5 (Phospho) | 24 h specific |