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. 2015 Mar 26;43(9):4505–4516. doi: 10.1093/nar/gkv176

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — AKT1 phosphorylates H3-T45 phosphorylation more efficiently than AKT2. AKT knockdown MCF10A cells were treated with 0.4 μg/ml ADR for 18 h. (A) Real-time PCR analysis of AKT mRNA. (B) Western blot analysis of total cell extracts with the indicated antibodies. (C) Phosphorylated H3-T45 ChIP assay of the CDKN1A locus. (D) Real-time PCR analysis of CDKN1A mRNA. (E) Real-time qPCR analysis of the indicated genes in ADR-treated MCF10A cells. Real-time qPCR and ChIP assay data shown are the average values of at least three independent experiments. Standard deviations are indicated as error bars. *P < 0.05, **P < 0.001.