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RHC-Glo splicing reporter minigene assay. Upper panel: schematic representation of the RHC-Glo splicing reporter minigene and the sub-cloned sequences. Lower panel: Agarose gel electrophoresis of RT-PCR minigene splicing products expressed in HEK293 cells. Inclusion or exclusion of exon 2 is indicated on the right. The gel pictures are representative results from two experiments.
