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. 2015 Apr 14;43(9):4531–4546. doi: 10.1093/nar/gkv327

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Neil1 protein and RNA abundance in mES and human prostate cancer cells with inherent or reduced levels of Rad9 protein. (A) Immunoblotting analyses used to measure indicated DDR proteins in Rad9^+/+ and Rad9^−/− mES cells. β-Actin, loading control. (B) Immunoblotting used to measure NEIL1 protein abundance in DU145 cells with inherent or shRAD9 knocked down RAD9 levels. β-Actin, loading control. (C) Same as B, but PC-3 cells were examined. (D) qRT-PCR used to assess Neil1 and Rad9 RNA levels in Rad9^+/+ and Rad9^−/− mES cells, plotted relative to β-Actin levels. (E, F) Same as D, but using DU145 and PC-3 cells, respectively, with inherent or shRNA-reduced levels of RAD9. Error bars in D, E and F represent standard deviation of three independent experiments.