Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 14;43(9):4531–4546. doi: 10.1093/nar/gkv327

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — NEIL1 promoter-luciferase reporter activity in DU145 and mES cells with inherent or reduced levels of RAD9. Chimeric constructs of the NEIL1 promoter-luciferase reporter are schematically represented on the Y-axis. The X-axis indicates luciferase activity as fold above values obtained for the promoterless vector, pGL-Basic. (A) Human NEIL1 promoter sequence. DU145 (dark bar), DU145-shRAD9 (light bar) host cells. (B) Mouse Neil1 promoter sequences. mES Rad9^+/+(dark bar), Rad9^−/− (light bar) host cells. Error bars represent the standard deviation of three independent experiments. Luc, luciferase. Numbers on constructs in Y-axis correspond to nucleotide positions in promoters relative to the start of transcription. Dark ovals represent intact p53-binding sites; light ovals indicate mutation sites. Pointed regions of promoters contain RAD9 binding sequences, as per the Chip-qPCR data, and are deleted.