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. 2015 Apr 14;43(9):4531–4546. doi: 10.1093/nar/gkv327

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Clonogenic survival of mES and DU145 cells with varying status of Rad9 after UV, menadione, and gamma-ray treatment. (A, B) Sensitivity of cells to 254 nm UV light. (C, D) Sensitivity of cells to menadione. (E, F) Sensitivity of cells to gamma rays. A, C and E, parental DU145 cells or those with shControl or shRAD9, and the latter with insertless pCMV6-AC-DDK-His vector, or ectopically expressing Rad9⁺ or NEIL1⁺. B, D and F, mES cells Rad9^+/+, Rad9^−/−, or the latter with insertless pCMV6-AC-DDK-His vector, or ectopically expressing RAD9⁺, Rad9⁺, NEIL1⁺ or Neil1⁺. Percent survival after each treatment was calculated as the number of colonies formed in treated versus mock-treated populations, times 100. Points are the average of three independent trials, each with two dishes per point. Error bars, standard deviation.