Figure 1.

A three-pronged iSAT approach for studying in vitro ribosome construction, where iSAT is an integrated method for the assembly of ribosomes from in vitro transcribed rRNA and subsequent translation by these ribosomes in the same compartment. Analyses to study these co-activated processes include measurements of mRNA transcription through use of a fluorescent RNA aptamer, measurements of protein translation through use of a fluorescent reporter protein and assessment of ribosome assembly through ribosome profiling.