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. 2015 Apr 20;43(9):4774–4784. doi: 10.1093/nar/gkv329

Figure 5.

Figure 5.

Ribosome profiling of iSAT reactions with and without crowding and reducing agents. Profiles represent A254 readings as relative signals versus gradient volume, in ml, measured from the top of 10–40% sucrose gradients, with peak identities labeled. For non-translating iSAT reactions (A–D), no reporter plasmid was added to 50 μl iSAT reactions including (A) no additives (gray, repeated in B–D), (B) 2% PEG 6000, (C) 2 mM DTBA or (D) 2% PEG 6000 and 2 mM DTBA. For translating iSAT reactions (E–H), pY71mRFP1-SpA was included as a reporter plasmid in 200 μl iSAT reactions including (E) no additives (gray, repeated in F–H), (F) 2% PEG 6000, (G) 2 mM DTBA or (H) 2% PEG 6000 and 2 mM DTBA.