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. 2015 Apr 20;43(9):4591–4601. doi: 10.1093/nar/gkv376

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Readthrough assay of systematic Sc-eRF1 L123 mutants. Readthrough frequencies of the systematic substitution mutants of Sc-eRF1 L123 are shown. S13-I01, S13-I03, S13-I05 and S13-I07 (Supplementary Table S1) assay strains that have UAA, UAG and UGA stop codons and UGG codon, respectively, were transformed with the wild type and L123 mutants of Sc-eRF1 on the p416GPD (URA3 marker) expression vector. Readthrough frequencies are indicated by percentage, where 100% readthrough was based on the results of a UGG reporter gene; values are indicated as mean ± SD from three independent measurements (see also Supplementary Table S3). A break in the vertical axis is indicated by a wave-line. Schematic drawing of the dual-luciferase readthrough assay constructs inserted at the HO locus of chromosome (Chr) IV of the assay strains is shown within the lower panel. Nucleotide residues for stop codons are shown in capitals and surrounding residues in lowercases.